A laboratory experiment for undergraduate biophysical chemistry is described, in which the acid concentration and temperature dependences of the decarboxylation of pyrrole-2-carboxylate are measured using a continuous ultraviolet (UV) spectrophotometric assay. Data collection and analysis are structured using principles of guided inquiry. Data…

Biochemists and life scientists in general are now encountering a paradox regarding the public image of their work; society demands and encourages pursuit of more knowledge but at the same time is suspicious and fears the novelties it brings. At this historical moment, we are experiencing a delicate balance between the birth of a new era and a…

Capillary electrophoresis is gradually working its way into the undergraduate laboratory curriculum. Typically, experiments utilizing this newer technology have been introduced into analytical or instrumental courses. The authors of this article have introduced an experiment into the introductory laboratory that utilizes capillary electrophoresis…

A collaborative-learning approach to teaching biochemistry to a diverse group of students that was developed is proving successful. This instructional strategy has increased student academic performance and changed the way in which students master science.

Mage is a graphics program developed for visualization of three-dimensional structures of proteins and other macromolecules. An application of the Mage program is reported here for developing interactive instructional graphics files (kinemages) of much smaller scale. Examples are given illustrating features of VSEPR models, permanent dipoles,…

An enzyme exercise to address the problem of students inability to visualize chemical reaction at the molecular level is described. This exercise is designed as a dry lab exercise but can be modified into a classroom activity then can be augmented by a wet lab procedure, thereby providing students with a practical exposure to enzyme function.

The use of a network-ready color camera is described which is primarily marketed as a security device and is used for experiments in developmental biology, genetics and biochemistry laboratories and in special student research projects. Acquiring and analyzing project and archiving images is very important in microscopy, electrophoresis and…

Emotionally charged experiences alter memory storage via the activation of hormonal systems. Previously, we have shown that compared with rats trained for a massed spatial learning task in the water maze in warm water (25 degrees C), animals that were trained in cold water (19 degrees C) performed better and showed higher levels of the stress…

Recognition memory, involving the ability to discriminate between a novel and familiar object, depends on the integrity of the perirhinal cortex (PRH). Glutamate, the main excitatory neurotransmitter in the cortex, is essential for many types of memory processes. Of the subtypes of glutamate receptor, metabotropic receptors (mGluRs) have received…

Nonreinforced retrieval can cause extinction and/or reconsolidation, two processes that affect subsequent retrieval in opposite ways. Using the Morris water maze task we show that, in the rat, repeated nonreinforced expression of spatial memory causes extinction, which is unaffected by inhibition of protein synthesis within the CA1 region of the…

Following initial encoding, memories undergo a prolonged period of reorganization. While such reorganization may occur in many different memory systems, its purpose is not clear. Previously, we have shown that recall of recent contextual fear memories engages the dorsal hippocampus (dHPC). In contrast, recall of remote contextual fear memories…

Since Congress passed .08 blood alcohol concentration (BAC) as the national standard for impaired driving in October 2000, 28 U.S. States including Indiana have enacted .08 BAC law. This study investigated perceived impact of the .08 law among Indiana residents and their attitudinal and perceptional changes since the enforcement of the law. The…

An inquiry-based laboratory course was created in an effort to increase student responsibility in learning and to improve teaching in areas related to molecular medicine. Authentic medical cases with both scientific and clinical aspects formed the basis of a project-oriented course that also included student laboratory work focused on the…

The topic of psychoactive drugs is one of inherent interest to college students. We used this insight to design and implement a multidisciplinary undergraduate course with psychoactive drugs as the central theme. The Medical Science of Psychoactive Drugs examines the biological mechanisms underlying all major effects of psychoactive drugs,…

Student assessment, in all its forms, is central to the educational process. In this paper in the series, "Bridging the Gap", I describe how assessment can be used as a powerful instrument for influencing how and what students learn and how and what instructors teach in a manner that is conducive to educationally sound curriculum change…