Traditional phenotyping methods are labor-intensive, time-consuming, and often destructive measurements that describe plant traits. Technology allows the advancement of sensor-based phenotype data to be collected digitally. This area of work is called high-throughput phenotyping (HTP). HTP is rapid, non-destructive, and non-invasive. The effective use and practical implementation of HTP technology in agriculture relies on an engaged workforce that understands plant breeding and emerging technology typically including unmanned aerial vehicle (UAV) technology. There are limited educational resources available on this topic that are an accessible and cost-effective solution for sharing new advancements. The open educational resource "UAV-based High-Throughput Phenotyping in Plant Breeding" was created to address this educational need and introduces HTP technology and the applications in plant breeding.