Introduces a laboratory experiment that isolates a bacterial polypeptide sample and measures the concentration of polypeptides in the sample. Uses Escherichia coli strain MM294 and performs a bio-rad assay to determine the concentration of polypeptides. (YDS)

Isotope substitution often affects the rate of an organic reaction and can be used to reveal the underlying mechanism. A series of experiments that use (super 1)H NMR to determine primary and secondary isotope effects, activation parameters, and the regioselectivity of butanone enolization are described.

An experiment using methyle methanethiosulfonate (MMTS) and phenylmethylsulfonyl flouride (PMSF) to specifically modify the cysteine and serine residues in the active sites of papain and subtilism respectively is demonstrated. The covalent modification of these enzymes and subsequent rescue of papain shows the beginning biochemist that proteins…

Various phenomena that are observed in the process of heating solid iodine in closed vessels at different pressures and temperatures are described. When solid iodine is heated in an evacuated ampoule where the pressure is less than 10(super -3), no noticeable color appears and immediate condensation of tiny iodine crystals is visible higher up on…

A convenient, inexpensive, environment friendly, and regioselective conversion of an aziridine to an oxazolidinone is developed by using iodide salt and CO[2] in water. A description is provided, on the way in which this series of experiments will show students how to change experimental conditions to obtain mainly one desired regiosomer of a…

The experiment involving enantiomeric resolution, as an illustration of chiral technology, is an excellent early organic chemistry lab experiment. The success of this enantiomeric resolution can be judged by melting point, demonstrated by [plus or minus]-mandelic acid-(1R,2S)-(--)-ephedrine system.

Drosophila melanogaster is a popular organism for studying genetics and development. Maintaining Drosophila on medium prepared with varying concentrations of beer and evaluating the effects on reproduction, life cycle stages and other factors is one of the exercises that is versatile and applicable to many student levels.

The spectrophotometric method assists in the study of potassium permanganate-oxalate reaction. Basic analytical techniques and rules are implemented in the experiment, which can also include the examination of other compounds oxidized by permanganate.

A chemical laboratory information profile (CLIP) of the chemical chlorine, produced in small quantities in the laboratory, is presented. The profile summarizes physical and harmful properties, exposure limits, reactivity risks, and symptoms of major exposure for the benefit of teachers and students using the chemical in the laboratory.

An experiment using a variable volume cell is presented where students actively control and directly observe the phase equilibrium inside the view cell. Measuring and exploring P-V-T phase behavior through dielectric constant measurements conveys the important concept that solvent behavior can be changed continuously in the sc fluid state.

The piezoelectric materials generate electricity or electric polarity in dielectric crystals when subjected to an applied voltage. The nanorings and nanobows are presented that can be used in nanoscale applications such as sensors, transducers, and electromechanical coupling devices.

The purpose of this study was to examine the relationship between laboratory behavioral measured impulsivity (using the Immediate and Delayed Memory Tasks) and suicidal attempt histories. Three groups of adults were recruited, those with either: no previous suicide attempts (Control, n = 20), only a single suicide attempt (Single, n = 20), or…

A number of laboratory exercises for the organic chemistry curriculum that emphasize enantioselective synthesis of the aldol condensation which involves the proline-catalyzed condensation between acetone and isobutyraldehyde are explored. The experiment illustrates some of the trade-offs involved in green chemistry like the use of acetone in large…

In a culture or society with high collectivism, contingent orientation and constrained autonomy are the prominent characteristics of adolescents' self-construal. This article examined whether Taiwanese adolescents' contingency and autonomy were associated with their prevalent preferences for buffet consumption. Findings in a panel survey indicated…

The analytical system to teach the subtleties of assaying alpha-dicarbonyl compounds in wine is described. Spectrophotometry is determined to be minimally useful in the experiment where the GC-MS (methyglyoxal) method proved to be ideal to confirm that the compounds analyzed in the wine samples were indeed the glyoxals based on the formation of…