Here, we propose a laboratory exercise to quickly determine single nucleotide polymorphisms (SNPs) in human "alcohol dehydrogenase 1B (ADH1B)" and "aldehyde dehydrogenase 2 (ALDH2)" genes involved in alcohol metabolism. In this exercise, two different genotyping methods based on polymerase chain reaction (PCR), namely…

Owing to the critical role of stereochemistry in biotechnology, medicine, and industry, it must be well represented in undergraduate lab curricula. To achieve these stereochemical laboratory requirements without sacrificing existing lab techniques, we modified two standard laboratory experiments to include chiral GC analysis as follows. (1) The…

After a time away from the classrooms and laboratories due to the global pandemic, the return to teaching activities during the semester represented a challenge to both teachers and students. Our particular situation in a Microbial Physiology course was the necessity of imparting in shorter time, laboratory practices that usually take longer. This…

Outreach science labs aim to promote students' interest. Previous research has often suggested that performing experiments in such labs has a positive effect on their interest. However, these studies often lack a comparison to the effects of performing them at school. This research gap was addressed in the present study. The sample consisted of…

Herein we describe an accessible and safe at-home experiment to separate lycopene from tomatoes via liquid-liquid extraction. As a qualitative experiment, it is a canonically elementary instance for high school students to digest the conception of miscibility, immiscibility, and density difference of the two solvents. This experiment was performed…

We are all users of lithium-ion batteries (LIBs), the electrochemical energy storage devices that revolutionized our life, making the diffusion of portable and wearable devices possible and now driving the switch from a combustion engine to electric vehicles in the transportation sector. These positive trends are however leading to two whiplash…

Undergraduate laboratory courses are vital for both enhancing student learning and preparing students for their future careers. Despite their importance, laboratory courses are often met with a lack of enthusiasm from students. For pre-health students specifically, laboratory courses are commonly seen as part of a check-list that needs to be…

Ethanol (EtOH) is among the most consumed drugs in the world. The behavior of humans after ingestion of this drug is characteristic: At low doses it may be excitatory and at higher doses, it may induce depressant/sedative effects. Similar effects are observed in the zebrafish experimental model ("Danio rerio"), which has about 70%…

Approximations can help to orient the student in an organic chemistry laboratory. There the student needs to develop the solvent systems for the reaction and isolation of organic reactants and products. The adage "like dissolves like" helps in this regard, but this approach is vague and entirely qualitative. More quantitative approaches…

Laboratory chemical synthesis research typically lacks the preplanned hazard responses found in production-scale industrial laboratories. Chemical safety management is a known challenge in education-based facilities, which is concerning for academic and national laboratory environments working with inexperienced student researchers. At the…

Stereoselective reduction of ketones to alcohols using baker's yeast is a classic experiment in the undergraduate laboratory. Here we illustrate the reduction of racemic ethyl 2-fluoro-3-oxobutanoate to the corresponding alcohol using a set of four commercially available ketoreductases (KREDs). The reaction sets two stereocenters with four…

A simple demonstration adaptable to classroom conditions is presented, which involves the preparation of raffle tickets using filter paper and the solution of selected fluorophores. With an appropriate dilution, the text written on the tickets is not visible to the naked eye; however, it becomes readable once put under a UV lamp. Besides being a…

Laboratory learning in the life sciences is historically centered around following recipe-like instructions to complete activities with defined outcomes. The American Association for the Advancement of Science's "Vision and Change" report has called for a change in science teaching. The report emphasizes the importance of providing…

A renewed experimental procedure for the unknown carbonyl experiment has been developed using hydrazone synthesis, thin layer chromatography (TLC) analysis, and melting point determination to differentiate between structurally similar ketones and aldehydes in undergraduate organic chemistry practical courses. Benzaldehyde, p-tolualdehyde,…

Intrinsic motivation plays a unique mediating role in student academic performance. An inquiry-based learning (IBL) physics practical is studied in terms of effects on secondary school students' intrinsic motivation towards performing science practicals. After performing IBL experiments on ionizing radiation, 38 secondary school physics students…