Discusses a laboratory technique of enzyme purification by affinity chromatography as part of an undergraduate biochemical methodology course. Provides preparation details of the rat muscle homogenate and reagents. Proposes column requirements and assaying information. (MVL)

Describes a laboratory technique for quantitative analysis of caffeine by an isotopic dilution method for coupled gas chromatography-mass spectroscopy. Discusses caffeine analysis and experimental methodology. Lists sample caffeine concentrations found in common products. (MVL)

Described are the purposes, laboratory set-ups, and procedures of four classroom experiments: ultrasound speedometer; vibrating-bar depth gauge; folding three-dimensional model of equipotential surfaces; and a simple optical system for the reconstruction of images from computer-generated holograms. Diagrams and pictures are provided. (YP)

Specific ways in which lectures, laboratories, cooperative group learning, and examinations and grading can be used as part of a strategy to increase pharmacy student interest and involvement in basic science are outlined. The techniques focus on making the presentation of content at least as important as the content itself. (MSE)

Activities that use dandelions to show the phenomena of geotropism and autotropism are described. Directions for collecting the stalks and observing the gravitropic response are included. The topics of lag time and bending rates, autotropism, growth rate changes, presentation time, and gravity detection are discussed. (KR)

Analyzes undergraduate general-chemistry laboratory manuals (n=10) and identifies illustrative verbs representative of the types of cognitive skills given in Bloom's Taxonomy. Finds that, although the majority of the manuals are similar in their lack of promoting higher-order cognition, a few newer manuals possess the requisite activities for…

Presents protocols for setting up a Winogradsky column and biofilm slides, interpreting the chemical transformations that occur in the column as a result of color changes in the soil, identifying common microorganisms, and determining the microbial composition of the column over specified intervals of time. (WRM)

The Applications in Biology/Chemistry (ABC) curriculum is a real-world approach designed for the middle 50% of secondary students who may not learn in a predominantly abstract way. Stressing contextual and cooperative learning, ABC features 10 laboratory exercises and other hands-on, problem-solving activities involving visits to job sites and…

Describes a biochemistry laboratory course in which the curriculum revolves around a single theme: the purification, characterization, and molecular biology of threonine dehydrogenase (TDH) from Escherechia coli. Lists examples of related class research projects. Contains 41 references. (WRM)

Discusses preliminary research on a new heuristic tool for learning from laboratory activities in secondary science. Finds that use of the science writing heuristic facilitated students in generating meaning from data; making connections among procedures, data, evidence, and claims; and engaging in metacognition. (Contains 29 references.)…

Describes the benefits of using calculator-based laboratory equipment in science classes. (WRM)

Scientific knowledge is tentative and open to revision. This sense of tentativeness, or changeability, is missing in the way textbooks and teachers often portray the nature of science (NOS). Instead, students are left with the impression that the scientific method produces sure knowledge and that they can uncritically accept the knowledge because…

This paper describes the author's experience of designing and teaching an interdisciplinary course titled "Light", in collaboration with a visual artist. The goals of the course, texts used, laboratory work, art studio work, and other course activities are discussed with an emphasis on how light was used as a theme to make connections between…

This paper describes some simple and rapid techniques for examining the growth responses of fungal hyphae cultivated on environmental gradients. The creation of such gradients using agar-based growth media in petri dishes is explained, along with recommendations for quantitative macroscopic and microscopic measurements. The intention is to provide…

Lab safety begins with the teacher. Teachers must make learning how to be safe an integral and important part of their professional development and work. Teachers who are unfamiliar with laboratory instruction should take whatever steps necessary to prepare for the unique challenges associated with safety in conducting laboratory investigations…