Laboratory exercise is helpful for medical students to understand the basic principles of molecular biology and to learn about the practical applications of molecular biology. We have designed a lab course on molecular biology about the determination of single nucleotide polymorphism (SNP) in human REV3 gene, the product of which is a subunit of…

Students' understandings of foundational concepts such as noncovalent interactions, pH and pK[subscript a] are crucial for success in undergraduate biochemistry courses. We developed a guided-inquiry activity to aid students in making connections between noncovalent interactions and pH/pK[subscript a]. Students explore these concepts by examining…

A procedure for the solution polymerization of styrene using di-"tert"-butyl peroxide (DTBP) as the initiator is described. The use of DTBP allows for end-group analysis by [superscript 1]H NMR spectroscopy and calculation of the number-average molecular weight of the polymer. This experiment was designed as a laboratory introduction to…

Traditional school laboratory exercises on a system of moving objects connected by strings involve deriving expressions for the system acceleration, a = (?F)/m, and sketching a graph of acceleration vs. force. While being in the form of rational functions, these expressions present great opportunities for broadening the scope of the analysis by…

This paper describes a student laboratory in the Unit Operations Laboratory at the Colorado School of Mines: air separation by pressure swing adsorption. The flexibility of the system enables students to study the production of enriched nitrogen or oxygen streams. Automatic data acquisition permits the study of cycle steps and performance.…

Teaching simple circuits and Ohm's law to students in the introductory classroom has been extensively investigated through the common practice of using incandescent light bulbs to help students develop a conceptual foundation before moving on to quantitative analysis. However, the bulb filaments' resistance has a large temperature dependence,…

A novel method of measuring the coefficients of kinetic and rolling friction is proposed. The method is simple to implement and reliable. Samples of measurements and calculations are presented.

Gas-phase nitrogen oxides are important in the formation of tropospheric ozone. NO[subscript x] (NO and NO[subscript 2]) as well as tropospheric ozone have been shown to have negative effects on human health. Therefore, accurately measuring NO[subscript x] concentrations in the atmosphere is important. In this laboratory experience, students…

This work describes a simple experiment to measure the resistor temperature as a function of the applied power and proves that it is an efficient way to introduce some important physical concepts in classroom, including the Joule's first law, hot-spot temperature, thermal resistance, thermal dissipation constant, time constant and the Newton's law…

The development and evaluation of a 6-hours laboratory class, based on capillary electrophoresis (CE) and the detection of microbial contaminants, is described. It can be easily scaled up or down, to suit class sizes up to 188 and completed in a shorter time scale. CE uses narrow-bore fused-silica capillaries to separate a complex array of large…

The separation of chamazulene from hydrophilic contaminants present in blue tansy oil provides a visually engaging example of two common techniques: extraction and thin-layer chromatography (TLC). This application uses liquid CO[subscript 2] as a lipophilic solvent to pull a brilliant blue hydrocarbon molecule, chamazulene, out of or through a…

The miniaturization of flow injection analysis devices presents several improvements related to analytical procedures. However, a cost reduction and simplification are required to allow extensive use in undergraduate teaching laboratories. Here we present a low cost microfluidic electroanalytical device composed only of interlocking toy blocks,…

This laboratory experiment leverages the pedagogical value and multidisciplinary nature of biodiesel production from vegetable oil to introduce students to continuous-flow chemistry, a modern and rapidly growing approach to chemical synthesis. An interdisciplinary approach exposes students to the practical and conceptual aspects of modern…

Expensive, highly reflective barium sulfate plates are typically used to obtain solid-state UV-vis electronic spectra. Through normal laboratory use these plates become dirty, chip, and break, thus requiring replacement. An alternative to purchasing new plates is to make your own by combining a mechanical stabilizer, such as gum arabic, with…

A teaching laboratory experiment is described where students prepare in vitro transcription reactions of a fluorescent RNA aptamer, named Broccoli, and observe the production of the aptamer in real-time on a fluorescence plate reader. Alternate visualization methods with minimal costs are also described for laboratories lacking this…