The COVID-19 pandemic presented numerous challenges for all levels of the education system. In March 2020, virtually all colleges and universities in the United States moved all instruction to an online environment as a result of the pandemic. While this posed challenges for all faculty, it posed a particular challenge for those faculty members…

This laboratory experiment describes the production and purification of plasmid DNA for undergraduate biochemistry and biotechnology courses. This experiment performed in a one-week period includes the protocols for plasmid pVAX1-LacZ production in "Escherichia coli" DH5[alpha] cells and subsequent purification of supercoiled pVAX1-LacZ.…

A six-laboratory session biofilm-focused biochemistry laboratory module is described. Through just six four-hour laboratory sessions over three weeks, students first master background knowledge and fundamental experimental techniques to explore questions involving bacterial biofilms at the interface of microbiology and biochemistry and then…

The study of gels and their properties is a compelling topic both technologically and scientifically, and should therefore be emphasized in chemistry and material science syllabuses. In the present laboratory experiment, we propose two experiences, aimed at introducing gelation and gel transport properties using silica gels made from sodium…

A laboratory exercise on the interaction between the herbicide pendimethalin (PM) and goat serum albumin (GSA), a carrier protein present in mammalian blood circulation, is described. Fluorescence spectroscopy was used to study the binding reaction between PM and GSA. Titration of a constant amount of the protein (GSA) with increasing ligand (PM)…

Next generation sequencing has revolutionized molecular biology and has provided a mechanism for rapid DNA and RNA sequence analysis. Yet, there are few resources to introduce next generation sequencing into the undergraduate biochemistry and molecular biology curriculum. Herein, we describe the design, execution, and assessment of a four-week…

This sequence of labs was developed for an upper level undergraduate cell biology course at Fairfield University. The labs are based on the use of the K562 human erythroleukemia cell line, a model system that is exceptionally amenable to an undergraduate cell biology lab course due to its ease of maintenance and propagation and usefulness for…

This activity introduces droplet-based millifluidics and the usage of aqueous droplets as independent chemical microreactors for reaction-kinetics studies. Students build their own droplet generators from common macroscopic glassware and connecting parts to create trains of millimeter-sized droplets in which a redox reaction takes place. The…

An approach for incorporating preliminary drug discovery research into a biochemistry laboratory is described. During a total of 42 hr (one 3-hr laboratory section a week for 14 weeks), students were exposed to bioinformatics; molecular cloning; protein expression, purification, and characterization; and enzymatic kinetic assays. This…

Advancements in computation and machine learning have revolutionized science, enabling researchers to address once insurmountable challenges. Bioinformatics, a field that heavily relies on computer-driven analysis of biological data, has greatly benefited from these developments. However, traditional bioinformatics instruction frequently lacks the…

Course-based undergraduate research experiences (CUREs) provide students with valuable opportunities to engage in research in a classroom setting, expanding access to research opportunities for undergraduates, fostering inclusive research and learning environments, and bridging the gap between the research and education communities. While…

Virtual laboratories (VLs) enable students to experiment, analyze data, or interact with digital content in a nonphysical space. VLs include simulations, electronic notebooks, videos, and augmented reality. As part of the "VL Project," comprising five academic institutions in Ireland, we sought to determine how VLs might enhance…

Reflection exercises have been used within anatomy education to evaluate empathy, humanism, and professionalism. A typical reflection exercise consists of directed prompts to guide the student's reflection as it relates to the experience and/or research question. The aim of this study was to utilize reflections through an open-ended format to…

This study examines a Technology, Pedagogy, Content Knowledge (TPACK) training framework for pre- and in-service mathematics teachers that enables them to experience inquiry-based learning as learners in a dynamic geometry environment that serves as a mathematics laboratory. We investigate the effect of this experience on the teachers' TPACK,…

Engaging in argument from evidence is a fundamental science practice. However, preservice elementary teachers have difficulty constructing arguments. They need effective experience with argument construction in science. Written argumentation is crucial in the process of science learning. One of the approaches for learning science through…