Objective: Define microbiological characteristics of pathogens causing lower urinary tract infections (LUTI), frequency of "Chlamydia trachomatis" (CT) or "Neisseria gonorrhea" (GC), and accuracy of self-diagnosis by college women with LUTI symptoms. Participants: Sexually active women with LUTI symptoms attending a large south-eastern university. Methods: Participants completed a 15-question Qualtrics™ survey, provided urine for urinalysis and culture and a self-collected vaginal swab for CT/GC testing. Results: "Escherichia coli" grew in 72.3% of cultures. Cultures showed 49.1% growing [greater than or equal to]10[superscript 5] and 46.1% between 10[superscript 4] and 10[superscript 5] colony forming units/mL (CFU/mL). Most pathogens (94.6%) were sensitive to nitrofurantoin. Three participants were positive for CT and the LUTI self-diagnosis accuracy was 72.0%. Conclusions: "Escherichia coli" was the primary uropathogen. Cultures with [greater than or equal to]10[superscript 4] CFU/mL identified 95.2% of LUTIs, and nitrofurantoin is the empiric drug of choice. CT is rarely identified in this population and college women are accurate making a self-diagnosis of LUTI.