This study aimed to explore the effects of integrating learning-related animations and anthropomorphic warm-colored multidimensional concept maps into multimedia learning materials to stimulate positive emotions in university students and to investigate whether these materials could improve their learning achievements. The research method employed a true experimental design. Participants were 158 volunteer students recruited from the College of Management at a national university in central Taiwan. Data analysis methods included analysis of covariance and regression analysis. Results showed that multimedia learning materials using learning-related animated multidimensional concept maps were more effective in stimulating students' positive emotions and improving their learning outcomes than the other three types. Notably, students' learning achievements were directly influenced by the use of different types of multimedia learning materials rather than through the mediation of positive emotions. In conclusion, this study demonstrates that integrating learning-related animations and anthropomorphic warm-colored multidimensional concept maps into multimedia learning materials can stimulate students' positive emotions and positively impact their learning achievements. This study innovatively integrates additional elements such as learning-related animations and intrinsic design elements like anthropomorphic warm-colored multidimensional concept maps into multimedia learning materials, providing additional contributions to the cognitive theory of multimedia learning and the principles of emotional design.