Background/purpose: Attitudes toward environmental conservation have become a key factor in addressing global ecological challenges. Positive environmental attitudes play a crucial role in adopting sustainable practices. This study aimed to assess the attitudes of university students from the Peruvian Amazon toward environmental conservation. Materials/methods: A quantitative, non-experimental, descriptive, and cross-sectional study was conducted with a sample of 588 male and female university students. Participants responded to an Ad Hoc questionnaire designed to evaluate attitudes toward environmental conservation, which demonstrated adequate internal consistency. Results: Most participants reported a very favorable (46.9%) or favorable (36.1%) attitude toward environmental conservation, while a smaller percentage showed an indifferent (11.9%), very unfavorable (2.7%), or unfavorable (2.4%) attitude. Regarding the dimensions, the cognitive aspect reflected the highest proportion of very favorable responses (61.9%), followed by the affective (50.3%) and behavioral (32.5%) dimensions. However, the behavioral dimension also showed a higher proportion of indifferent responses (16.3%). Statistically significant differences were found in attitudes toward environmental conservation according to the year of study. Conclusion: University students from the Peruvian Amazon exhibit generally positive attitudes toward environmental conservation, particularly in the cognitive and affective dimensions. Nevertheless, a gap is evident in the behavioral dimension, as these attitudes do not always translate into concrete actions.