Apurinic/apyrimidinic (AP or abasic) sites are deoxyribonucleic acid (DNA) lesions that result from the loss of a nucleobase by hydrolysis of the "N"-glycosyl bond. AP sites are the most frequent lesions in cells and are caused by environmental and cancer therapeutic genotoxins. Herein, the bicinchoninic colorimetric assay, typically…

NMR spectroscopy is a powerful spectroscopic tool that not only allows the determination of structures after synthesis, as usually explained to students of Organic Chemistry, but also is useful in many other fields such as medicine imaging, real-time industrial processes, material sciences, or metabolomics. In this experiment, students performed a…

For single-slit diffraction and double-slit interference experiments, commercially made slits can be the most expensive parts, especially since the prices of laser pointers have become very low. One option is to use a razor blade to cut slits in either paint or electrical tape on microscope slides. However, this takes practice, and there is some…

In this paper I describe an interactive Gauss's law computer simulation using the GlowScript programming environment. The simulation calculates a point charge's electric field at locations on the surface of a Gaussian cube and displays the electric field in GlowScript's 3D graphics window. The point charge can be moved, and the electric field…

A straightforward experimental set-up, requiring a two-liter bottle, a ruler and a stopwatch, is used to provide data appropriate for modelling with Torricelli's Law in the simplest case, and a more sophisticated differential equation when losses are taken into account and a pipe extension is considered. With only an exit hole included in the…

Calculating analytical uncertainties as a part of method validation is a relevant aspect of field and laboratory practices in instrumental analytical chemistry subjects, which usually require complex algorithms. This work describes the development and didactic use of an automatic and straightforward informatics tool, implemented in an Excel macro,…

As a model study for improving undergraduate organic chemistry laboratories with contemporary research concepts and practices, we evaluated a project module aiming at the synthesis of two porphyrin compounds. The module was implemented in a format that differed in several ways from that of a traditional course. It was designed for small student…

Background: The adoption of virtual laboratories (VLs) has experienced a significant surge, catalysed by the global changes in the educational landscape, notably influenced by the COVID-19 pandemic. Originally supplementary to traditional in-person laboratory experiments, VLs swiftly transitioned into a primary source of hands-on laboratory…

Alcohol consumption has profound effects on behavior, such as impaired judgment, addiction or even death. It is estimated that alcohol contributes to around three million deaths worldwide, 13.5% of them in young people with ages between 20 and 39 years. Consequently, it is necessary to raise awareness among college and high school students of the…

An effective, practical approach for incorporating molecular biology tools to biology and biochemistry students is described. For 6 weeks, students are exposed to bacterial transformation, plasmid DNA purification and analysis, parasite transfection, ectopic expression of fluorescent proteins, fluorescence microscopy, and flow cytometry analysis.…

The chemical experiment in this study is specifically created for undergraduate students studying energy and the environment. The target of the experiment is for students to comprehend the deactivation mechanism of the Ce/TiO[subscript 2] catalyst by oxides of phosphorus through practical experiments and enhance their knowledge of environmental…

The utilization of virtual compound libraries and virtual screening has become a routine method in drug discovery and has accelerated the research of pharmacy and translational medicine. With the development of computer-aided drug design (CADD), it is essential to incorporate virtual experiments into the education of medicinal chemistry. Herein,…

We describe a three-day advanced organic laboratory experience that includes the solid-phase synthesis of an unknown three amino acid polypeptide for junior/senior undergraduate chemistry majors. Students conduct a series of 1D and 2D NMR experiments (HSQC, HMBC, TOCSY) and analyze the data to identify the side chain residues of the peptide and…

Many students find introductory general chemistry courses difficult because they feel alienated by traditional approaches to teaching and learning. This can become particularly problematic in laboratory sessions where students simply follow processes and procedures that students can view as being mundane and lacking creativity. Contextualised…

Protein molecular weight standards are routinely employed in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to estimate the apparent sizes of unknown proteins within a sample. For training students, a laboratory course based on the production of marker proteins is proposed. Following fractionation by column chromatography, a…