Biotechnology has great importance as a socioscientific issue in STEM education. This study aimed to explore the knowledge and attitudes of college students toward biotechnology. A total of 236 university students participated in the study. Questionnaires on knowledge and attitudes towards biotechnology were used for data collection. The results revealed that participants had inadequate knowledge about several basic concepts related to genetics and biotechnology, particularly those concerning genetically modified organisms. The results regarding the attitudes revealed more neutral results. The participants had a neutral attitude towards biotechnology, with mean scores ranging from 2.71 to 3.55. They were generally against buying genetically modified products but supported biotechnology for medical purposes and strongly desired to increase their knowledge about genetically modified products. The participants did not have a negative opinion of biotechnology in general but were critical of its purpose and use. The correlation analysis between knowledge and attitudes produced weak correlations. Offering supplementary resources on biotechnology can enhance students' and individual's understanding and attitudes of this topic.